Personalized 3D-Bioprinted Skin Constructs Based on Patient-Derived Decellularized ECM for Improved Wound Healing and Tissue Regeneration
발표자
강래희 (포항공과대학교)
연구책임자
이준민 (포항공과대학교)
초록
내용
The skin, the body’s largest organ, is vital for protection and homeostasis, making it a focus of regenerative medicine. Yet, most models lack patient specificity and fail to reflect the native extracellular matrix (ECM). Here, we introduce a three-dimensional (3D) bioprinted skin model using patient-derived decellularized ECM (pddECM) and keratin–alginate (KA) bioinks. The pddECM enhanced dermal fibroblast viability and collagen I expression, while KA supported keratinocyte activation. The model improved cell migration and angiogenesis under reduced hypoxia. Cytokine profiling showed increased intercellular adhesion molecule-1 (ICAM-1) and complement C5, with reduced interleukin-4 (IL-4) and interleukin-8 (IL-8). In vivo, gelatin methacryloyl (GelMA) with or without pddECM accelerated wound closure and preserved dermal tissue. This patient-specific platform holds promise for regenerative wound care and drug testing.