[3PS-074]
Personalized 3D-Bioprinted Skin Constructs Based on Patient-Derived Decellularized ECM for Improved Wound Healing and Tissue Regeneration
발표자강래희 (포항공과대학교)
연구책임자이준민 (포항공과대학교)
Abstract
The skin, the body’s largest organ, is vital for protection and homeostasis, making it a focus of regenerative medicine. Yet, most models lack patient specificity and fail to reflect the native extracellular matrix (ECM). Here, we introduce a three-dimensional (3D) bioprinted skin model using patient-derived decellularized ECM (pddECM) and keratin–alginate (KA) bioinks. The pddECM enhanced dermal fibroblast viability and collagen I expression, while KA supported keratinocyte activation. The model improved cell migration and angiogenesis under reduced hypoxia. Cytokine profiling showed increased intercellular adhesion molecule-1 (ICAM-1) and complement C5, with reduced interleukin-4 (IL-4) and interleukin-8 (IL-8). In vivo, gelatin methacryloyl (GelMA) with or without pddECM accelerated wound closure and preserved dermal tissue. This patient-specific platform holds promise for regenerative wound care and drug testing.