[3PS-052]
Development of Carboxymethylated Silk Fibroin Microgels with Anisotropic Structures for 3D Printable Cell Guidance Scaffolds
발표자유연우 (서울대학교)
연구책임자이기훈 (서울대학교)
Abstract
Microgel assemblies have high porosity and a reversible frictional force, making them a promising form for 3D printing in the biomedical field. Because most microgels are isotropic and spherical, which have limitations in guiding cell alignment, recent studies have focused on developing anisotropic microgels for cell alignment. Silk fibroin, a natural fibrous protein, has excellent biocompatibility and high mechanical strength, making it an attractive candidate for 3D printing inks.
In this study, we fabricate anisotropic silk fibroin microgels through carboxymethylation. Silk fibroin directly modified to keep anisotropic structures. Carboxymethylated silk fibroin (CMSF) was 3D printable, and aligned along the printing direction. To stabilize the printed structure, they were frozen and immersed in an acidic solution, maintaining their shape in the liquid phase. In this result, CMSF inks are expected to be used as a 3D printable cell guidance scaffold.